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. 2022 May 14;12:7989. doi: 10.1038/s41598-022-11533-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Bright field and (b) fluorescence image of B. cinerea conidia treated for 10 min with Cu6-PLGA NPs. NPs fluorescence signal was evident in germinated B. cinerea conidia (white square) and non-germinated ones. (c) 3D reconstruction of B. cinerea conidia treated with NPs for 10 min. The fluorescence signal was detected inside conidia, along the whole thickness of the spore. (d) Bright field and (e) fluorescence image of B. cinerea germ tube. The NPs fluorescence signal was well visible in the expanding germ tube after 10 min of administration. (f) Bright field and (g) fluorescence image of B. cinerea conidium and hypha. The fluorescence signal was well visible both in the conidium and the hypha after 1 h of administration. (h) Overlap of the bright field image and the fluorescence image which shows the localization of Cu6-PLGA NPs inside the fungal conidium and hypha.