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. 2022 May 14;12:8008. doi: 10.1038/s41598-022-11760-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of UBE2C in the tissues and cell lines of the EC. (A) Expression of UBE2C in the EC. (B) Expression of UBE2C in normal esophageal tissues. (C) Expression of UBE2C in esophageal cancer cell line kyse150. (D) Expression of UBE2C in esophageal cancer cell line Eca109. (E) Expression of UBE2C in esophageal cancer cell line TE-1. (F) Expression of UBE2C in HEEC (normal esophageal cell line) . (G) Expression of UBE2C in the tissue microarray assay of 150 patients with esophageal cancer. (H) Differential expression analysis of UBE2C in cancer and adjacent tissues in the tissue microarray assay. Paired sample t-test was used to compare cancer and adjacent samples. ^***P < 0.001. (I) Subject operating characteristic (ROC) curve analysis and area under curve (AUC) statistics were used to evaluate the ability of UBE2C to distinguish esophageal cancer from adjacent normal tissues.(AUC:0.879).