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. Author manuscript; available in PMC: 2022 Oct 13.
Published in final edited form as: Oncogene. 2022 Apr 13;41(20):2824–2832. doi: 10.1038/s41388-022-02307-9

Figure 2. Stable expression of KIF20A in androgen-dependent prostate cancer cells confers castration resistant growth in vitro.

Figure 2.

(A) LNCaP (N = 5), (B) LAPC4 (N = 4), and (C) VCaP (N = 2) cells were stably transduced with KIF20A or Empty Vector (EV) control. Androgen-deprived cells were cultured in 5% charcoal-stripped serum (CSS). Numbers of live (trypan blue excluded) LNCaP and LAPC4 cells were plotted over time. Because VCaP cells were difficult to dissociate, cell proliferation was determined by crystal violet staining after 12 days of culture. Eluted stain was measured by absorbance at 560 nm. Unpaired t-tests measured statistical significance. Representative cell images at 10X magnification were taken on Day 8 of proliferation. (Image contrast was increased for better visualization after image capture for LAPC4 or VCaP EV and KIF20A cells.) ** p < 0.01; **** p < 0.0001. Error bars: SEM