Skip to main content
. Author manuscript; available in PMC: 2022 Nov 5.
Published in final edited form as: Nat Cell Biol. 2022 May 5;24(5):685–696. doi: 10.1038/s41556-022-00906-y

Fig. 6: CEN is required for distant cebpd activation and fluid regulation after cardiac injury.

Fig. 6:

a, Schematic of CEN deletion. b, Schematic of CM ablation in wild-type or CEN−/− fish and tissue collection for cebpd ISH. c, ISH indicating normal cebpd induction in injured CEN−/− hearts. n = 6 animals for all groups. Arrows indicate ISH signals (violet). d, qPCR for cardiac cebpd RNA levels. n = 4 CreER− and 5 CreER+ biologically independent samples for CEN+/+ animals, and n = 6 CreER− and 5 CreER+ biologically independent samples for CEN−/− animals. Five hearts were pooled for each biological sample. Mean ± S.E.M., unpaired two tailed t-test. e, ISH indicating no cebpd induction in CEN−/− brains after cardiac injury. n = 22 CreER− and 20 CreER+ animals for CEN+/+, and n = 18 CreER− and 16 CreER+ animals for CEN−/−. Black arrows indicate ISH signals. Red arrows indicate loss of ISH signals. f, ISH indicating no cebpd indcution in CEN−/− kidneys by cardiac injury. Arrows as in (e). n = 8 CreER− and 9 CreER+ animals for CEN+/+, and n = 6 CreER− and 4 CreER+ animals for CEN−/−. g, Schematic of agonist treatment and tissue collection for cebpd ISH. h, ISH indicating that CEN is required for cebpd induction in brain by GR or MR agonists. n = 34 (vehicle), 36 (dexamethasone), 40 (spironolactone) animals for CEN+/+. n = 13, 8, and 17 animals for CEN−/−. Arrows as in (e). Abbreviations as in Fig.5. i, ISH indicating that CEN is required for cebpd induction in kidney by GR or MR agonists. Animal numbers as in (h). j, Schematic of experiments assessing fluorescent dye transfer into vasculature. k, Fluorescence images indicating inefficient dye transfer in injured CEN−/− animals. l, Quantification of experiments in (k). Single trial with n = 9 animals for both CEN+/+ groups, and n = 11 animals for CreER− and 10 animals for CreER+ in CEN−/− groups. Mean ± SEM, unpaired two-tailed t-test. Scale bars in c, e, f, h, i: 100 μm. Scale bars in k: 500 μm.