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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Nat Cell Biol. 2022 May 9;24(5):748–756. doi: 10.1038/s41556-022-00897-w

Fig. 2: Male and female soma show reduced CENP-A in contrast to oocytes.

Fig. 2:

a, Mating scheme to test memory in the soma in the F1 generation. b, Bone marrow metaphase spreads (control and F1 generation) are representative of both male and females: each pair of CENP-A foci represents sister centromeres in mitosis. c, Quantification of CENP-A foci intensities in control (grey) and F1 (purple) generations in male and female soma combined. N = 642, 684 centromeres (top to bottom). **/* P<0.0001/P< 0.05, Mann-Whitney U test (two-tailed). d, Pooled male and female CENP-A intensities from Fig. 2c, replotted with male and female separated to show that both contain weakened centromeres. N = 251, 433, 390, 251 centromeres (top to bottom). e, Quantification of CENP-A chromatin showing weakened centromeres in the soma compared to oocytes from the same female. Two independent experiments (1, 2) are shown, each comparing a single F1 animal (from H × H cross) to controls. N = 112, 44, 94, 51, 169, 89, 98, 68 centromeres (top to bottom). **/*= P<0.001/P<0.01, Mann-Whitney U test (two tailed). Error bars: median ± 95% CI. Scale bars: 5 μm (main panel), 1μm (inset). Source numerical data are available in source data.