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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Nat Cell Biol. 2022 May 9;24(5):748–756. doi: 10.1038/s41556-022-00897-w

Extended Data Fig. 2. Weakened centromeres in the male germline are independent of meiotic stage.

Extended Data Fig. 2

Because oocytes were analyzed at metaphase I and spermatocytes at prophase I (Fig. 1), we confirmed that F1 spermatocytes also show weakened centromeres at metaphase I. Images (a) and quantification (b) of F1 spermatocytes show CENP-A reduced to a similar level at metaphase I (70.54 ± 7.1% of control) as prophase I. Each of the CENP-A foci represents four centromeres (a pair of homologous chromosomes, each with two sisters). N = 330 (control), 284 (F1 progeny). Scale bars: 5 μm (main panel), 1μm (inset). Quantification of SYCP3 foci from the same cells (c) shows no decrease (114.90 ± 5.6% of control). N = 235 (control), 259 (F1 progeny). ** P<0.001, Mann-Whitney U Test (two tailed). Error bars: median ± 95% CI. Source numerical data are available in source data.