TABLE 1.

Immunohistochemistry protocols.

Primary antibody	Antigen retrieval	Secondary Ab/amplification
Rabbit Anti-CD34 (Abcam, ab81289)	Heat Induced at 75 °C for 20 min in 1 mM EDTA, pH 8.0	Vectastain Elite ABC HRP Kit (Vector Laboratories, PK-6200)
Rabbit Anti-Ang1 (Abcam, ab102015)	Heat induced at 95 °C for 10 min in 10 mM sodium citrate, pH 6.0	Vectastain Elite ABC HRP Kit (Vector Laboratories, PK-6200)
Rabbit Anti-VEGF (Abcam, ab46154)	Digestion in 0.5 mg/mL hyaluronidase for 60 min at 37 °C	EnVision + HRP labelled polymer solution (Dako, K4002)
Mouse Anti-Col III (Sigma-Aldrich, C7805)	Digestion in 0.4 mg/mL protease K in 30 mM Tris HCl for 4 min at RT, 0.5mg/mL Hyaluronidase for 60 min at 37 °C, and 0.5 N acetic acid for 4 h at 4 °C	Secondary (BD Biosciences, 550331), ABC amplification (Vector Laboratories, PK-6200)
Rabbit Anti-TNFα (Novus Biologics, NBP1-19532)	Digestion in 0.5 mg/mL Pepsin in 0.1 N HCl for 20 min at RT	Secondary (Jackson Co. 111-035-003)
Rabbit Anti-MMP13 (Abcam, ab39012)	Digestion in 0.5 mg/mL Hyaluronidase for 60 min at 37 °C	Secondary (BD Sciences, 550338), ABC amplification (vector laboratories, PK-6200).