Fig. 6.

Enhanced cellulase production by selected mutants. a Final validation of the selected mutants for cellulase production by flask fermentation. Data were normalized to the wild-type strain SHY169. b SDS-PAGE analysis of secreted cellulases in the supernatants of the selected mutants. c Comparison of fermentation performance of the best strain R5-2 and the wild-type in 5-L fermenter. Data are the average mean of triplicate samples, and the error bars represent standard deviations. Wet cell weight was measured as follows. Ten mL fermentation broth sample was centrifuged at 8000 g for 10 min in pre-weighed tube. The supernatant was removed. The wet weight of the pellet was measured and calculated to the unit g/L