Fig. 3.

In vivo evaluation of tumor targeting and immune responses. a In vivo fluorescence images to reveal the biodistribution of Zr-TCPP(TPP)/R837 and Zr-TCPP(TPP)/R837@M post i.v. injection into 4T1-tumour-bearing mice at the indicated time points. White circles indicate tumours; b the accumulation curve of Zr-TCPP(TPP)/R837 and Zr-TCPP(TPP)/R837@M in the tumour tissue by measuring the fluorescence intensity of tumours at different time points post i.v. injection, error bars are based on SD (n = 3); c, d DC maturation in the tumour-draining lymph nodes induced by various treatments on mice bearing 4T1 tumours, as assessed by flow cytometry after staining with CD11c, CD80, CD86 and live dead; e–g cytokine levels of TNF-α, IL-6 and IL-12p70 in sera from mice after different treatment. h, i Flow cytometry plots and the corresponding quantification showing percentages (gated on CD4⁺ cells) of CD4⁺Foxp3⁺ Treg cells in tumours tumors after various treatments indicated. Data are expressed as means ± SD (n = 3). Statistical significances were calculated via one-way ANOVA Tukey's multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001