Table 2.

Oligonucleotides used in this study

Primers	Sequence (5’–3’ with restriction sites underlined)
HpaB-NdeI-F	GGGCCTCATATGAAACCAGAAGATTTCCGCGCCAG
HpaB-SpeI-R	GGGCCCACTAGTTATTTCAGCAGCTTATCCAGCATGTTGA
HpaC-NdeI- F	GGGTTTCATATGCAATTAGATGAACAACGCCTGCGC
HpaC-SpeI-R	GGGCCCACTAGTTAAATCGCAGCTTCCATTTCCAGCATCA
pZM1-HpaC-F	CTAGCGAAAGGAGGAGTTGACAATTAATCATCGGCTCGTATAATGTGTGGA
pZM1-HpaC-R	AGAGTTTGTAGAAACGCGTCGACTCCTC
MatB-F-BamHI	CCGGGATCCAAAGGAGGACAACCATGAGCAACCATCTTTTCGACG
MatB-R	CGACCTCCTTTAGCACCTTACGTCCTGGTATAAAGATCGGC
MatC-F	CGTAAGGTGCTAAAGGAGGTCGAAGATGGGTATTGAATTACTGTCCATAGG
MatC-R-MfeI	CCGCAATTGTCAAACCAGCCCGGGC
pEKEx3-MatBC-F1	AGCGCATTGTTAGATTTCATACACGG
pEKEx3-MatBC-F	CAGGAACCGTAAAAAGGCCGCGTTG
pEKEx3-MatBC-R	TTGTAGAAACGCAAAAAGGCCATCC
pEKEx3-MatBC-R2	TGAAATCTAACAATGCGCTCATCGTCA
pZM1-HpaBC-F	CGGCCTTTTTACGGTTCCTGTTGTAGAAACGCGTCGACTCCTCC
pZM1-HpaBC-R	GCCTTTTTGCGTTTCTACAATCCCAGTCACGACCCTAGGG