Table 4.
Primers used for Real-time RT-PCR
| Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| β-actin | GGAGATTACTGCCCTGGCTCCTA | GACTCATCGTACTCCTGCTTGCTG |
| P16INK4A | AACACTTTCGGTCGTACCC | GTCCTCGCAGTTCGAATC |
| Rb1 | CATGCTAGCAGCTGTTCTTTAC | AGTTTGCCTATATCAGCACAGT |
| ALDOA | TGTTGTGGGCATTAAGGTAGA | GAGGGACGAGGGAGTATGC |
| ENO1 | CAAGTCCTTCATCAAGAACTATCC | TGTCAGGTCATCTCCCACAAT |
| HK1 | TCTAAACTCTGGGAAACAAAGGT | GAAGTCAATCAGGATGTTACGG |
| HK2 | GCCGTAGTGGACAAGATAAGAGAG | GCCTCCATACCCATCTTGCTAC |
| LDHA | TCCCTGAAGTCTCTGAACCCG | TCCTCCTTGATTCCATAGAGACC |
| LDHB | GGTGGTGGACAGTGCCTATGA | GACTTCGTTCTCGATGCCGTA |
| PFKM | GTGAAAGACCTGGTGGTTCAGA | CTCTGAGATCTTGCCTTCCTTC |
| PK | GAAACAGCCAAAGGGGACTA | GGACTCCGTCAGAACTATCAAA |
| PGAM1 | GAGCGACACTATGGGGGTCTAAC | TCTGCGTACCTGCGGTCCTT |
| TPI1 | CACTCTGAACGCAGCCAAGC | CACCCAGGTAGCTCCTAAGTCC |