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. 1999 Feb;65(2):680–685. doi: 10.1128/aem.65.2.680-685.1999

TABLE 2.

Maltase and maltose permease activities of baker’s yeast strains L38 and NL67 and their transformantsa

Strain Maltase activityb
Permease activityc
Mal Gal Eth Glu Mal Gal Eth Glu
L38 2,000 60 140 2.1 2,100 32 21 0.3
NL67 3,000 1,000 1,700 14 5,100 1,100 2,800 2.2
L38 + BEJ17 1,800 40 130 1.4 2,000 26 23 0.5
L38 + MALx3–VH1 1,800 49 73 1.2 2,000 20 19 0.3
L38 + MALx3–VH7 1,900 540 630 32 2,100 370 610 1.3
L38 + MALx3–VH50 1,800 860 1,200 80 2,000 680 920 1.6
L38 + MALx3–VH23 2,900 60 130 1.3 3,800 20 20 0.2
NL67 + BEJ17 3,100 980 1,600 6.0 5,200 1,100 2,800 1.9
NL67 + MALx3–VH1 3,200 600 1,200 5.0 5,200 640 2,000 1.9
NL67 + MALx3–VH7 3,000 1,500 1,900 57 5,200 2,200 3,100 3.8
a

Strains and transformants were grown to mid-log phase in maltose (Mal), galactose (Gal), ethanol (Eth), or glucose (Glu). Values shown are means of data derived from three experiments. Assays were carried out in triplicate with standard errors of less than 10% for maltase results and less than 15% for maltose permease results. 

b

In nanomoles of p-nitrophenol released per minute per milligram of protein. 

c

In picomoles of [14C]maltose taken up per minute per milligram (dry weight) of yeast cells.