TABLE 2.
Maltase and maltose permease activities of baker’s yeast strains L38 and NL67 and their transformantsa
| Strain | Maltase activityb
|
Permease activityc
|
||||||
|---|---|---|---|---|---|---|---|---|
| Mal | Gal | Eth | Glu | Mal | Gal | Eth | Glu | |
| L38 | 2,000 | 60 | 140 | 2.1 | 2,100 | 32 | 21 | 0.3 |
| NL67 | 3,000 | 1,000 | 1,700 | 14 | 5,100 | 1,100 | 2,800 | 2.2 |
| L38 + BEJ17 | 1,800 | 40 | 130 | 1.4 | 2,000 | 26 | 23 | 0.5 |
| L38 + MALx3–VH1 | 1,800 | 49 | 73 | 1.2 | 2,000 | 20 | 19 | 0.3 |
| L38 + MALx3–VH7 | 1,900 | 540 | 630 | 32 | 2,100 | 370 | 610 | 1.3 |
| L38 + MALx3–VH50 | 1,800 | 860 | 1,200 | 80 | 2,000 | 680 | 920 | 1.6 |
| L38 + MALx3–VH23 | 2,900 | 60 | 130 | 1.3 | 3,800 | 20 | 20 | 0.2 |
| NL67 + BEJ17 | 3,100 | 980 | 1,600 | 6.0 | 5,200 | 1,100 | 2,800 | 1.9 |
| NL67 + MALx3–VH1 | 3,200 | 600 | 1,200 | 5.0 | 5,200 | 640 | 2,000 | 1.9 |
| NL67 + MALx3–VH7 | 3,000 | 1,500 | 1,900 | 57 | 5,200 | 2,200 | 3,100 | 3.8 |
Strains and transformants were grown to mid-log phase in maltose (Mal), galactose (Gal), ethanol (Eth), or glucose (Glu). Values shown are means of data derived from three experiments. Assays were carried out in triplicate with standard errors of less than 10% for maltase results and less than 15% for maltose permease results.
In nanomoles of p-nitrophenol released per minute per milligram of protein.
In picomoles of [14C]maltose taken up per minute per milligram (dry weight) of yeast cells.