TABLE 3.
Melibiase (MEL1) and β-galactosidase (LacZ) activities of PB1 transformants grown in maltose, galactose, or glucosea
| Strain | MEL1 activityb (MALx2)
|
LacZ activityc (MALx1)
|
||||
|---|---|---|---|---|---|---|
| Mal | Gal | Glu | Mal | Gal | Glu | |
| PB1 + YRp7 | 2.0 | 0.8 | ND | 7.0 | 0.1 | ND |
| PB1 + MALx3–VH1 | 350 | 1.1 | ND | 88 | 0.2 | ND |
| PB1 + MALx3–VH7 | 340 | 26 | 7.4 | 86 | 17 | 0.9 |
| PB1 + MALx3–VH9 | 360 | 4.0 | ND | 93 | 1.0 | ND |
| PB1 + MALx3–VH23 | 510 | 1.4 | ND | 140 | 0.2 | ND |
| PB1 + MALx3–VH50 | 330 | 61 | 22 | 81 | 35 | 4.4 |
a
Values shown are means of data derived from three experiments. Assays were carried out in triplicate. Standard errors were less than 10%.
b
In nanomoles of p-nitrophenyl released per minute per milligram of protein. Mal, maltose; Gal, galactose; Glu, glucose; ND, not detected.
c
In nanomoles of o-nitrophenyl released per minute per milligram of protein.