TABLE 4.
Fermentation activity in relation to maltase and maltose permease activities in recombinant strains of baker’s yeasta
| Strain | Gassingb in:
|
Maltase activityc | Permease activityd | |
|---|---|---|---|---|
| 1st h | 2nd h | |||
| L38 + BEJ17 | 180 | 52 | 30 | 12 |
| L38 + MALx3–VH1 | 180 | 51 | 25 | 10 |
| L38 + MALx3–VH7 | 200 | 130 | 270 | 280 |
| L38 + MALx3–VH23 | 180 | 69 | 19 | 10 |
| NL67 + BEJ17 | 220 | 210 | 380 | 1,600 |
| NL67 + MALx3–VH1 | 210 | 210 | 270 | 1,300 |
| NL67 + MALx3–VH7 | 220 | 220 | 400 | 1,700 |
| L38 + PDC1 | 200 | 120 | 31 | 1,800 |
| L38 + PDC1–VH7 | 210 | 180 | 270 | 1,900 |
| L38 + MALx3–VH50 | 220 | 170 | 390 | 530 |
Values shown are means of data received from three experiments. Fermentation activity determinations were carried out in duplicate with standard errors of less than 10%. Assays were carried out in triplicate with standard errors of less than 10% for maltase results and less than 15% for maltose permease results.
Milliliters of CO2 produced per gram (dry weight) of yeast.
In nanomoles of p-nitrophenol released per minute per milligram of protein.
In picomoles of [14C]maltose taken up per minute per milligram (dry weight) of yeast cells.