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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Biomaterials. 2021 Sep 1;277:121113. doi: 10.1016/j.biomaterials.2021.121113

Figure 3. Integrin-binding (cRGD) and syndecan-binding (AG73) peptide lead to morphological differences and improved adhesion in 2D.

Figure 3.

A) Representative immunostaining images of primary human NP cells per condition, red is actin, blue is nuclei, scale bar is 50μm. B-C) Cell spreading and circularity behaviors of single cells cultured upon peptide functionalized alginate hydrogel. D) Percent single cell attachment cultured upon hydrogel conditions. E,F) Representative immunostaining images of paxillin and phospho-Myosin Light Chain (pMLC), scale bar is 50 μm. G) Cell clustering behavior upon peptide functionalized alginate hydrogels. H) Quantification of paxillin rich focal adhesions per cell per condition. I) Quantification of pMLC protein expression. n ≥ 6 unique hydrogels from 3 human donors. Error bars are ±SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, using a one-way ANOVA with Tukey's multiple comparison's test (B, C, D, H, I).