FIG. 5.

Agarose gel electrophoresis of λ DNA digested with partially purified enzyme preparations from LM0230(pND805), showing the effects of Mg²⁺, ATP, and Ado-Met on pND805 endonuclease activity. Lane 1, λ HindIII standard marker; lanes 2 to 5, preparations containing partially purified enzyme extract from LM0230(pND805); lanes 6 to 9, preparations containing extract from LM0230(pSA3); lanes 2 and 6, extract plus Mg²⁺; lanes 3 and 7, extract plus Mg²⁺ plus ATP; lanes 4 and 8, extract plus Mg²⁺ plus ATP plus Ado-Met; lanes 5 and 9, extract plus Mg²⁺ plus Ado-Met. The reaction mixtures were incubated for 1 h at 37°C.