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. Author manuscript; available in PMC: 2023 Apr 14.
Published in final edited form as: Cell. 2022 Apr 14;185(8):1431–1443.e16. doi: 10.1016/j.cell.2022.03.023

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Myc-Tag (9B11) Mouse mAb Cell-Signaling Technology Cat#2233
PE anti-DYKDDDDK Tag Antibody Biolegend Cat#637310
Chemicals, peptides, and recombinant proteins
In-Fusion Snap Assembly Master Mix with Competent Cells Takara Cat#638951
N-Acetyl-L-cysteine (NAC) Sigma-Aldrich Cat#A9165
Human AB Serum Valley Biomedical Inc. Cat#HP1022
Recombinant human IL-2 protein NCI BRB Preclinical Repository https://ncifrederick.cancer.gov/research/brb/
Mirus Trans-IT Lenti Transfection Reagent Mirus Cat#MIR6606
ddPCR Supermix for Probes (No dUTP) Bio-rad Cat#1863024
Critical commercial assays
RosetteSep Human CD4+ T Cell Enrichment Cocktail STEMCELL Technologies Cat#15062
RosetteSep Human CD8+ T Cell Enrichment Cocktail STEMCELL Technologies Cat#15063
IL-2 Human Instant ELISA Kit Thermo Fisher Cat#BMS221INST
Experimental models: Cell lines
LentiX 293T Clontech Cat#11131D
K562 myelogenous leukemia cells ATCC Cat#CCL-243
E6-1 Jurkat T cells ATCC Cat#T1B-152
K562 CD19 (CD19 extracellular domain_myctag_hPDGFRtransmembrane) Roybal et al., 2016 N/A
K562 GFP Roybal et al., 2016 N/A
K562 ALPPL2 Hyrenius-Wittsten et al, 2021 N/A
K562 TRE ALPPL2 Hyrenius-Wittsten et al, 2021 N/A
MDA-MB-468 ATCC Cat#HTB-132
U87 MG ATCC Cat#HTB-14
U87EGFRviii Choe et al., 2021 N/A
M28MESO Hyrenius-Wittsten et al, 2021 N/A
M28MESO/ALPPL2KO Hyrenius-Wittsten et al, 2021 N/A
SK-OV-3 UCSF Cell and Genome Engineering Core Cat#CCLZR377
SK-OV-3ALPPL2 Hyrenius-Wittsten et al, 2021 N/A
Experimental models: Organisms/strains
NOD scid IL-2Rγ−/− (NSG) (8–12 weeks) Jackson Laboratories Cat#005557
Oligonucleotides
hTERT Probe: 5′-(SUN)-TCAGGACGTCGAGTGGACACGGTG-(ZEN/IBFQ)-3′ IDT N/A
HIV-1 Ψ Probe 5′-(FAM)-ATCTCTCTCCTTCTAGCCTC-(ZEN/IBFQ)-3′ IDT N/A
hTERT Forward Primer: 5′-GGCACACGTGGCTTTTCG-3′ IDT N/A
hTERT Reverse Primer: 5′-GGTGAACCTCGTAAGTTTATGCAA-3′ IDT N/A
HIV-1 Ψ Forward Primer: 5′-TACTGACGCTCTCGCACC-3′ IDT N/A
HIV-1 Ψ Reverse Primer: 5′-TCTCGACGCAGGACTCG-3′ IDT N/A
Recombinant DNA
pHR_SFFV Addgene ID#79121
pHR_PGK Addgene ID#79120
pHR_Gal4UAS_PGK_mCherry Addgene ID#79124
pHR_Gal4UAS_tBFP_PGK_mCherry Addgene ID#79130
Software and algorithms
Prism Version 9 Graphpad N/A
FlowJo V10.7.1 FlowJo LLC N/A
Jalview Jalview N/A
Incucyte Software v2018B Sartorius N/A
QuantaSoft Bio-Rad N/A