REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP	ThermoFisher Scientific	A18811; RRID: AB_2535588
Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	ThermoFisher Scientific	21445; RRID: AB_2535862
F(ab')2-Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP	ThermoFisher Scientific	A24470; RRID: AB_2535939
Bacterial and virus strains
DH5-α	ThermoFisher Scientific	18265017
Stbl2™	ThermoFisher Scientific	10268019
Biological samples
Serum/Plasma from donors	This paper	N/A
Chemicals, peptides, and recombinant proteins
Ficoll-Hypaque	GE Healthcare	17-1440-03
Nunc-Immuno™ MicroWell™ 96 well solid plates	MERCK	M9410
Falcon® 96-well Clear Round Bottom TC-treated Cell Culture Microplate	CORNING	353077
Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well	ThermoFisher Scientific	15502
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein, Biotinylated	SinoBiological	40589-V08B1-B
ELISA Wash Buffer	ThermoFisher Scientific	N503
BSA Blocker	ThermoFisher Scientific	37525
eBioscience™ TMB Solution	ThermoFisher Scientific	00-4201-56
SuperSignal™ ELISA Pico Chemiluminescent Substrate	ThermoFisher Scientific	37069
DMEM/High glucose without L-glutamine, sodium pyruvate	Cytiva	SH30081
RPMI 1640	CORNING	10-040
L-Glutamine	CORNING	25-005
HyClone Penicillin-Streptomycin 100X solution	Hyclone	SV30010
Cosmic Calf Serum	Hyclone	SH30087
Fetal Bovine Serum	Hyclone	SH30071
Critical commercial assays
Zeba™ Spin Desalting Columns	ThermoFisher Scientific	89892
Easy-Titer™ Human IgG (H+L) Assay Kit	ThermoFisher Scientific	23310
Stop Solution for TMB Substrates	ThermoFisher Scientific	N600
PolyJet™ In Vitro DNA Transfection Reagent	SignaGen	SL100688
Luciferase Cell Culture Lysis 5X Reagent	Promega	E1531
Luciferase Assay System	Promega	E1500
Experimental models: Cell lines
293T cells	ATCC	CRL-3216
FcγRIIa and FcγRIIIa, CD4+ Jurkat reporter cell lines	Promega	G7010
Recombinant DNA
SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-FLAG tag (Codon Optimized)	SinoBiological	VG40589-CF
Human SARS coronavirus (SARS-CoV) Spike glycoprotein Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tag	SinoBiological	VG40150-CF
Human coronavirus (HCoV-OC43) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag	SinoBiological	VG40607-CF
Human coronavirus(HCoV-NL63) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag	SinoBiological	VG40604-CF
Human coronavirus(HCoV-229E) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag	SinoBiological	VG40605-CF
Software and algorithms
Gen5	Agilent	https://www.biotek.com/products/software-robotics-software/gen5-microplate-reader-and-imager-software/
FlowJo	Tree Star Inc.	v10
PRALINE multiple sequence alignment	IBIVU	https://www.ibi.vu.nl/programs/pralinewww/help.php
Adobe Illustrator	Adobe	25.4.1
GraphPad Prism	GraphPad Software	8.4.3
R	R Foundation	4.0.4
R Studio	R Foundation	1.4.1103