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. 2022 May 2;13:872188. doi: 10.3389/fphar.2022.872188

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Copyright © 2022 Zhou, Zhang, Jiang, Hu, Meng, Li, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Osteogenesis is enhanced under naringenin-modulated macrophage coculture medium. (A) Schematic illustration. (B,D) ARS staining and its quantification. (C,E) ALP staining and its quantification. (F) Transcript levels of osteoclast markers Acp5, CtsK, and Dc-stamp. (G, H) Western blot and optical density analysis of RUNX2 and OCN with GAPDH as reference. n = 4 per group. Data are expressed as mean ± SD. * # p < 0.05; ** ## p < 0.01; *** ### p < 0.001. * compared with con group; # compared with LPS + RAW group.