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. 2022 Apr 11;19(4):740–752. doi: 10.7150/ijms.69807

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Effect of NAC on VCM-induced increased expression of KIM-1 and NGAL in HK-2 cells and rat kidneys. The effects of NAC (A, B, C) and vitamin C (D, E, F) on the expression of KIM-1 and NGAL in HK-2 cells using western blot and semiquantified analysis. (G, H) KIM-1 expression and analysis in kidney tissue detected using immunohistochemistry. (I, J) NGAL expression and analysis in kidney tissue detected using an immunohistochemical technique. Data was performed from three independent experiments or 5 rats and presented as mean ± SEM. ^*p<0.05, significantly higher than that in the control group. ^#p<0.05, significantly lower than that in the VCM group. Significance was analyzed by ANOVA followed by Tukey's test.