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. 2022 Apr 11;19(4):740–752. doi: 10.7150/ijms.69807

Figure 4.

Figure 4

Effect of NAC on VCM-induced cell apoptosis in HK-2 cells and rat kidneys. (A, B, C, D) The expression and analysis of Bax, Bcl-2 and caspase-3 detected and analyzed by western blot in HK-2 cells treated with VCM and NAC or vitamin C. (E) Caspase-3 expression and analysis using immunofluorescence staining in HK-2 cells treated with VCM and NAC or vitamin C. (F) Caspase-3 expression and analysis using immunofluorescence staining in rat kidneys treated with VCM and NAC or vitamin C. Data was performed from three independent experiments or 5 rats and presented as mean ± SEM. *p<0.05, **p<0.01, significantly higher than that in the control group. #p<0.05, significantly lower than that in the VCM group. Significance was analyzed by ANOVA followed by Tukey's test.