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SDS-PAGE of 5-Oxoprolinase. Electrophoresis was done under the following conditions. A sample (about 30 μg protein) was loaded onto a 7.5% polyacrylamide gel after denaturation with 4% SDS and 10% mercaptoethanol. After electrophoresis, the protein was stained with Coomassie brilliant blue R-250. Markers: phosphorylase b (94 kDa), bovine serum albumin (67 kDa), ovalbumin (43 kDa), and carbonic anhydrase (30 kDa).
