TABLE 2.
Summary of purification of the 5-oxoprolinase from A. faecalis N-38A
| Purification step | Total amt of protein (mg) | Total activity (mU) | Sp act (mU/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cell extract | 612,000 | 133,000 | 0.22 | 1 | 100 |
| 40–80% (NH4)2SO4 precipitation | 391,000 | 119,000 | 0.30 | 1.4 | 89.5 |
| Phenyl-Toyopearl 650M | 1,350 | 70,800 | 52.4 | 238 | 53.2 |
| DEAE-Toyopearl 650M | 99.7 | 111,000 | 1,110 | 5,050 | 83.5 |
| DEAE-Toyopearl 650M | 65.8 | 97,500 | 1,480 | 6,730 | 73.3 |
| Butyl-Toyopearl 650M | 28.5 | 62,600 | 2,200 | 10,000 | 47.1 |
| DEAE-Toyopearl 650M | 15.9 | 35,000 | 2,200 | 10,000 | 26.3 |
| Sephadex G-100 | 10.3 | 22,100 | 2,150 | 9,770 | 16.6 |