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. 1999 Feb;65(2):712–717. doi: 10.1128/aem.65.2.712-717.1999

TABLE 2.

Summary of purification of the 5-oxoprolinase from A. faecalis N-38A

Purification step Total amt of protein (mg) Total activity (mU) Sp act (mU/mg) Purification (fold) Yield (%)
Cell extract 612,000 133,000 0.22 1 100
40–80% (NH4)2SO4 precipitation 391,000 119,000 0.30 1.4 89.5
Phenyl-Toyopearl 650M 1,350 70,800 52.4 238 53.2
DEAE-Toyopearl 650M 99.7 111,000 1,110 5,050 83.5
DEAE-Toyopearl 650M 65.8 97,500 1,480 6,730 73.3
Butyl-Toyopearl 650M 28.5 62,600 2,200 10,000 47.1
DEAE-Toyopearl 650M 15.9 35,000 2,200 10,000 26.3
Sephadex G-100 10.3 22,100 2,150 9,770 16.6