FIGURE 6.

NLRP3 signaling participated in DNLA-mediated neuroprotection. WT mice and NLRP3-KO mice were given DNLA (40 mg/kg) intragastrically for 14 consecutive days. On the seventh day after DNLA treatment, mice received a single intranigral injection of LPS (2 μg) on both sides of the hippocampus. After DNLA treatment for 10 days, the novel object recognition test (A) and Y-maze test (B) were determined. The neuronal number in hippocampus CA1 was measured by immunofluorescence staining; the red color indicated NeuN-positive cells, and the blue represented the DAPI-stained nucleus. Scale bar = 50 μm (C). Quantitative analysis of the number of NeuN-positive cells (D).*p < 0.05 compared with the WT control group; ^# p < 0.05 compared with the WT LPS-treated group.