FIGURE 7.

DNLA suppressed pyroptosis through inhibition of NLRP3 signaling. After DNLA treatment for 14 days, the cell membrane structure of the hippocampal neurons was discerned using the scanning electron microscope (A). Protein expressions of caspase-1 and NLRP3 in hippocampus CA1 were measured by immunofluorescence staining. The green color indicates caspase-1-positive cells, and red color indicated GSDMD-N-positive cells. Scale bar = 50 μm (B). Quantitative analysis of protein density of GSDMD-N (C) and caspase-1(D). *p < 0.05 compared with the WT control group; ^# p < 0.05 compared with the WT LPS-treated group. Data were expressed as mean ± SEM (n = 3).