Figure 2.

Schematic of the cellular localization of the two lipids products downstream of PIKfyve activity, PI(3,5)P₂ and PI(5)P, as well as the precursor PI(3)P. Due to the lack of suitable and/or sufficiently sensitive bioprobes, the localization of endogenous levels of PI(3,5)P₂ and PI(5)P is inferred from the localization of PIKfyve. PI(3,5)P₂ localizes on early endosomes, late endosomes and lysosomes. PI(5)P may also be present at all or some of these locations. CME: Clathrin-mediated endocytosis. CIE: Clathrin-independent endocytosis. EE: Early endosome. LE: Late endosome. MVB: Multivesicular body. EA: Endoplasmic reticulum. ERC: Early recycling compartment. TGN: Trans-Golgi network.