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Visualization of invasive cells. (A) Cells that invaded the ECM mimetic gel layer and crossed the membrane were stained with crystal violet. Red arrows on representative images indicate invaded cells, which were quantified (red numbers). Scale bar is 100 μm. (B) Quantitative analysis of images. Statistics: two-way ANOVA (Mg, Mg–6Ag compared to glass control = *) with Tukey's multiple comparison test (n = 12). * = p < 0.05; *** = p < 0.001; **** = p < 0.0001.
