Table 3.
VEGF release under hypoxia. HUVEC were incubated with conditioned media from the coculture (CC) or Saos-eGFP (MCS) and RF Fibroblasts (MCF) in monocultures on Mg-based materials or material without cells. Fold changes of VEGF concentration (relative to cell culture medium) are shown as the mean ± SD from two experiments with three samples (n = 6). Statistics: one-way ANOVA (sample vs medium control) with Dunn's multiple comparison test. **** = p < 0.0001.
| Day | conditioned medium CC | conditioned medium MCS | conditioned medium MCF | conditioned medium mat. control | |
|---|---|---|---|---|---|
| glass control | 1 | 0.97 ± 0.04 | 1.01 ± 0.25 | 0.99 ± 0.01 | 1.00 ± 0.00 |
| 3 | 0.96 ± 0.02 | 6.41 ± 4.07 **** |
1.03 ± 0.01 | 1.00 ± 0.00 | |
| 7 | 0.99 ± 0.02 | 1.19 ± 0.10 | 1.03 ± 0.02 | 1.00 ± 0.00 | |
| Mg | 1 | 1.00 ± 0.01 | 1.01 ± 0.02 | 1.00 ± 0.01 | 1.02 ± 0.03 |
| 3 | 0.97 ± 0.03 | 0.98 ± 0.03 | 0.99 ± 0.02 | 0.99 ± 0.04 | |
| 7 | 0.98 ± 0.02 | 0.89 ± 0.09 | 0.99 ± 0.04 | 1.02 ± 0.04 | |
| Mg–6Ag | 1 | 1.00 ± 0.01 | 1.01 ± 0.03 | 1.00 ± 0.01 | 1.01 ± 0.04 |
| 3 | 0.96 ± 0.03 | 0.98 ± 0.04 | 1.01 ± 0.02 | 0.99 ± 0.07 | |
| 7 | 0.99 ± 0.01 | 0.89 ± 0.05 | 1.02 ± 0.02 | 1.02 ± 0.03 |