TABLE 2.

Reproducibility. The benchmark sample NA24385 was selected for intra-assay reproducibility evaluation, whereas NA24694 was included in all three independent workflows for inter-assay reproducibility evaluation. Coefficients of variation (CV) of quality metrics are reported.

Reproducibility	Samples	SNVs			Indels
		Precision	Sensitivity	F-measure	Precision	Sensitivity	F-measure
	NA24385	0.9970	0.9918	0.9944	0.9969	0.9948	0.9959
	NA24385-2	0.9963	0.9914	0.9939	0.9952	0.9920	0.9936
Intra-assay	Mean	0.9967	0.9916	0.9941	0.9961	0.9934	0.9947
	SD	0.0005	0.0003	0.0004	0.0012	0.0020	0.0016
	CV (%)	0.0501	0.0322	0.0411	0.1221	0.1991	0.1607
	NA24694	0.9992	0.9993	0.9993	0.9968	0.9980	0.9974
	NA24694-2	0.9994	0.9993	0.9993	0.9974	0.9986	0.9980
Inter-assay	NA24694-3	0.9988	0.9989	0.9988	0.9963	0.9968	0.9966
	Mean	0.9991	0.9991	0.9991	0.9968	0.9978	0.9973
	SD	0.0003	0.0002	0.0003	0.0005	0.0009	0.0007
	CV (%)	0.0312	0.0235	0.0271	0.0551	0.0909	0.0726

SD, standard deviation; CV, coefficient of variation.