Figure 1.

AMPK-α is cleaved during apoptosis

(A and B) Jurkat cells were treated with SNG (3 μM) or anti-Fas (25 ng) for the indicated times and western blot analysis carried out.

(C) Caspase-3 activity assays were performed; data shown are mean ± SD (n = 3; ^∗p < 0.05 and ^∗∗∗p < 0.001 versus respective control).

(D) Cell viability was assessed; data shown are mean ± SD (n = 3; ^∗∗∗p < 0.001 versus respective control).

(E) Jurkat cells were treated with SNG (3 μM) or anti-Fas (25 ng) for 4 h, and DNA fragmentation assay was performed.

(F) Apoptosis was assessed by annexin V-FITC/PI staining; data in the right-hand panel show mean ± SD (n = 3) for the percentage of cells positive for both annexin V binding and PI staining (^∗∗∗p < 0.001).

(G) Jurkat (6 h), Molt-4 (8 h), HeLa (24 h), or HT-29 (24 h) cells were treated with the indicated concentrations of etoposide, and western blot analysis was carried out.