Table 3.
Overview of applied 3D imaging methods per cancer subtype.
| Cancer | Experimental model | Imaging method | Study |
|---|---|---|---|
| Bladder | Patient material, FFPE or fresh | 3D‐reconstructed 2D tissue sections | Jansen et al (2019) |
| Light sheet | Tanaka et al (2017); Tanaka et al (2018); Tanaka et al (2020) | ||
| Brain | In vivo models | Confocal | Lagerweij et al (2017) |
| Intravital | Barretto et al (2011); Ricard and Debarbieux (2014); Osswald et al (2015); Lagerweij et al (2017); Alieva et al (2017); Alieva et al (2019); Venkataramani et al (2019); Murty et al (2020); Xie et al (2021) | ||
| Breast | Patient material, FFPE or fresh | Confocal | Chen et al (2019) |
| Light sheet | Tanaka et al (2020) | ||
| Super‐resolution | Creech et al (2017) | ||
| 3D‐reconstructed 2D tissue sections | Booth et al (2015) | ||
| In vivo models | Intravital | Zomer et al (2013); Harney et al (2015); Zomer et al (2015); Beerling et al (2016); Harper et al (2016); Entenberg et al (2018); You et al (2018); Ilina et al (2020); Kondo et al (2021) | |
| Light sheet | Dobosz et al (2014) | ||
| Confocal | Tiede et al (2021) | ||
| Spectral confocal | Rios et al (2019) | ||
| Organoids | Light sheet | Tanaka et al (2020) | |
| Live 3D imaging | preprint: Dekkers et al (2021) | ||
| Spectral confocal | Dekkers et al (2020) | ||
| Colon / intestine | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2017); Tanaka et al (2020) |
| Confocal | Liu et al (2013) | ||
| 3D‐reconstructed 2D tissue sections | Korehisa et al (2018) | ||
| Organoids | Light sheet | Tanaka et al (2020) | |
| Live 3D imaging | Bolhaqueiro et al (2019); Schnalzger et al (2019); Badder et al (2020); Ponsioen et al (2021) | ||
| Fixed and live 3D imaging | Krotenberg Garcia et al (2021) | ||
| In vivo models | Intravital | Marangoni et al (2013); Arlauckas et al (2017); Fumagalli et al (2017); Fumagalli et al (2020) | |
| Head and neck | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2017); Si et al (2019) |
| Organoids | Live 3D imaging | preprint: Dekkers et al (2021) | |
| Kidney | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2020) |
| Multispectral confocal | van Ineveld et al (2021) | ||
| Organoids | Spectral confocal | Schutgens et al (2019); Calandrini et al (2020) | |
| Liver | Patient material, FFPE or fresh | Light sheet, confocal | Yoshizawa et al (2020) |
| Lung | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2017); Tanaka et al (2020) |
| 3D‐reconstructed 2D tissue sections | Yagi et al (2020) | ||
| In vivo models | Light sheet | Viallard et al (2020) | |
| Lymphoma | Patient material, FFPE or fresh | Confocal | Nojima et al (2017) |
| In vivo models | Intravital | Cazaux et al (2019) | |
| Ovarian | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2017); Tanaka et al (2020); Kastelein et al (2020) |
| Pancreatic | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2017); Noe et al (2018) |
| Confocal | Lin et al (2016) | ||
| In vivo models | Confocal | Messal et al (2021) | |
| Confocal | Lin et al (2016); Shen et al (2019) | ||
| Intravital | Conway et al (2018) | ||
| Prostate | Patient material, FFPE or fresh | 3D‐reconstructed 2D tissue sections | Tolkach et al (2018) |
| Confocal | Van Royen et al (2016); Verhoef et al (2019) | ||
| Skin | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2020) |
| In vivo models | Confocal | Fiore et al (2020) | |
| Intravital | Zomer et al (2015); Hirata et al (2015); Park et al (2019) | ||
| Soft tissue sarcoma | In vivo models | Intravital | Tang et al (2019) |
| Thyroid | Patient material, FFPE or fresh | Light sheet | Tanaka et al (2020) |