Figure 3. Nature and timing of SARS‐CoV‐2‐induced gene expression changes in pDCs.

• A, B
  Waterfall plots illustrating gene expression changes in pDCs 48 h post SARS‐CoV‐2 exposure, relative to mock‐treated cells, from two donors; D^high (A) and D^low (B), indicating genes with > 2 fold change in red (upregulated) and blue (downregulated).
• C, D
  Heat maps and unsupervised hierarchical cluster analyses of the > 2‐fold upregulated genes in D^high (C) and D^low (D).
• E
  To validate the wave pattern of gene expression, CXCL10 mRNA was quantified using RT‐qPCR in multiple pDC donors (n = 3–4) at different time points post TLR7 agonist R837, TLR3 agonist poly(I:C), and SARS‐CoV‐2 (1 MOI) exposure at indicated time points.

Data information: Bars represent mean values and equal symbols represent equal donors.