Figure 4. SARS‐CoV‐2 sensing and inflammatory cytokine induction by pDCs is mediated predominantly via MyD88.

• A, B
  Using CRISPR/Cas9, MyD88 knock‐out (KO) and AAVS1^KO (control) pDCs were generated. MyD88 protein levels in KO and control pDCs were analyzed by western blotting (A) and cellular DNA was sequenced to perform an Inference of CRISPR Edits (ICE) analysis (B).
• C, D
  MyD88^KO and control pDCs were either mock treated (mock) or exposed to SARS‐CoV‐2 (SARS‐2, 1 MOI), supernatants were collected at indicated time points and analyzed for type I IFNα (C) and CXCL10 (D). Done with n = 4.
• E, F
  Type I IFNα production was then determined in cell culture supernatant from SARS‐CoV‐2 exposed TRIF^KO or TRIG + MyD88^KO (E) and RIG‐I^KO or RIG‐I + MyD88^KO (F) pDCs. Done with n = 2.

Data information: Bars represent mean values and equal symbols represent equal donors. Statistical significance was determined using Mann–Whitney one‐tailed t test in (C) as MyD88‐KO data had no variance and values of 0. For (D) a Mann–Whitney two‐tailed T test was used for comparison between conditions of mock and MyD88 KO donors.

Source data are available online for this figure.