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. 2022 Apr 11;41(10):e108898. doi: 10.15252/embj.2021108898

Figure 6. UPF1LL activity is enhanced by translational repression.

Figure 6

  1. Framework for investigation of effects of translation inhibition on UPF1LL activity.
  2. RNA‐seq analysis of HEK‐293 cells identifies populations of genes that decreased in abundance with puromycin treatment (50 µg/ml for 4 h) and were rescued by UPF1LL‐specific knockdown. Indicated are genes that increased in abundance at least 1.4‐fold (FDR < 0.05) with UPF1LL‐specific knockdown under normal conditions.
  3. RT–qPCR analysis of indicated transcripts following transfection of HEK‐293 cells with indicated siRNAs and treatment with 50 µg/ml puromycin for 4 h. Relative fold changes are in reference to vehicle‐treated, NT siRNA. Black dots represent individual data points, and error bars indicate mean ± SD (n = 3 biological replicates). Dashed lines indicate log2 (fold change) of ± 0.5. PTC+ indicates the use of primers specific to transcript isoforms with validated poison exons (Lareau et al, 2007; Ni et al, 2007). See also Dataset EV3 for P‐values associated with each statistical comparison.

Source data are available online for this figure.