Figure 1. Temporal metabolomics reveals the mammalian metabolome is rapidly reshaped by iron depletion.

1. Schematic of temporal metabolomics workflow in human ARPE19 cells.
2. Multivariate modelling of the metabolome reveals distinct transitions over time (states I–IV) in response to iron chelation using deferiprone (DFP). OPLS‐DA was computed and generated from combined LC‐MS and GC‐MS datasets from three independent biological experiments each with four technical replicates per timepoint in each experiment.