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. 2022 Apr 22;41(10):e109191. doi: 10.15252/embj.2021109191

Figure 7. WT UPF3A can rescue NMD in full extent. Deletion on exon 4 disrupts functionality in both paralogs.

Figure 7

  1. Schematic representation of the UPF3A and UPF3B protein domains. Below are the respective mutated rescue constructs.
  2. Western blot analysis of WT and UPF3B KO clone 90 with Luciferase and UPF3A KDs respectively. Monitored expression of the FLAG‐tagged UPF3A and UPF3B rescue construct shown in (A). Rescue construct protein levels were detected with anti‐FLAG, anti‐UPF3A, and anti‐UPF3B (AK‐141) antibodies. Tubulin serves as control (n = 1). The asterisk indicates unspecific bands.
  3. Quantitative RT–PCR of the samples from (B). For RSRC2 and SRSF2, the ratio of NMD isoform to canonical isoform was calculated. Data points and means are plotted as log2 fold change (log2FC) (n = 3).

Source data are available online for this figure.