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. 2022 May 2;13:849742. doi: 10.3389/fimmu.2022.849742

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Copyright © 2022 Hosgood, Elliott, Jordan and Nicholson

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nanostring nCounter Organ Transplant Panel analysis of 4 cortex samples of kidneys comparing transcriptional analysis of differential expressed genes after 1h NMP compared to baseline pre samples. Total RNA was isolated and processed. (A) Heatmap of the top 25 significantly differentially expressed genes. (B) Volcano plot represents differential gene expression after 1h NMP. Difference in gene expression level with p-value adjusted <0.01 or <0.05 are marked by red dots. (C) Top 5 pathways up-regulated after NMP. Statistical significance was calculated by Mann Whitney test (*, p<0.05). (D) Principal component analysis on the top 100 differentially expressed genes.