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. 2022 Apr 8;101(7):101909. doi: 10.1016/j.psj.2022.101909

Table 1.

Virulence-associated genes: target genes, primers, sequences, PCR conditions, amplicon size, gene function, and references.

Target gene Primers Sequence (5ˊ→3ˊ) PCR conditions Amplicon size (bp) Campylobacter species or gene function References
16S - rRNA MD16S1
MD16S2
F: ATCTAATGGCTTAACCATTAAAC
R: GGACGGTAACTAGTTTAGTATT
35 cycles:
95°C – 30 s
59°C – 90 s
72°C – 60 s
857 Campylobacter genus Denis et al., 1999; Linton et al., 2000
mapA MDmapA1
MDmapA2
F: CTATTTTATTTTTGAGTGCTTGTG
R: GCTTTATTTGCCATTTGTTTTATTA
589 Campylobacter jejuni Denis et al., 1999
ceuE ceuE1
ceuE2
F: AATTGAAAATTGCTCCAACTATG
R: TGATTTTATTATTTGTAGCAGCG
462 Campylobacter coli Denis et al., 1999
flaA flaAF
flaAR
F: GGATTTCGTATTAACACAAATGGTGC
R: CTGTAGTAATCTTA AACATTTTG
30 cycles:
94°C – 60 s
48°C – 60 s
72°C – 60 s
1700 encodes the major flagellin protein (FlaA) http://campynet.vetinst.dk/Fla.htm
cadF F2B
R1B
F:TGGAGGGTAATTTAGATATG
R: CTAATACCTAAAGTTGAAAC
30 cycles:
94°C – 60 s
54°C – 60 s
72°C – 60 s
400 encodes an adhesion and fibronectin-binding protein involved in the process of invasion Konkel et al., 1999