Table 1.
Target gene | Primers | Sequence (5ˊ→3ˊ) | PCR conditions | Amplicon size (bp) | Campylobacter species or gene function | References |
---|---|---|---|---|---|---|
16S - rRNA | MD16S1 MD16S2 |
F: ATCTAATGGCTTAACCATTAAAC R: GGACGGTAACTAGTTTAGTATT |
35 cycles: 95°C – 30 s 59°C – 90 s 72°C – 60 s |
857 | Campylobacter genus | Denis et al., 1999; Linton et al., 2000 |
mapA | MDmapA1 MDmapA2 |
F: CTATTTTATTTTTGAGTGCTTGTG R: GCTTTATTTGCCATTTGTTTTATTA |
589 | Campylobacter jejuni | Denis et al., 1999 | |
ceuE | ceuE1 ceuE2 |
F: AATTGAAAATTGCTCCAACTATG R: TGATTTTATTATTTGTAGCAGCG |
462 | Campylobacter coli | Denis et al., 1999 | |
flaA | flaAF flaAR |
F: GGATTTCGTATTAACACAAATGGTGC R: CTGTAGTAATCTTA AACATTTTG |
30 cycles: 94°C – 60 s 48°C – 60 s 72°C – 60 s |
1700 | encodes the major flagellin protein (FlaA) | http://campynet.vetinst.dk/Fla.htm |
cadF | F2B R1B |
F:TGGAGGGTAATTTAGATATG R: CTAATACCTAAAGTTGAAAC |
30 cycles: 94°C – 60 s 54°C – 60 s 72°C – 60 s |
400 | encodes an adhesion and fibronectin-binding protein involved in the process of invasion | Konkel et al., 1999 |