Skip to main content
. 2022 Apr 22;25(5):104279. doi: 10.1016/j.isci.2022.104279

Figure 3.

Figure 3

miR-25 reguletes autophagy pathway during BCG and Mtb infection

(A–F) THP-1 cells pre-transfected with control mimic, miR-25 mimic, control inhibitor, miR-25 inhibitor NPC1 siRNA, or NFKBIZ siRNA were infected with BCG or H37Rv for a specified period of time, western blotting of the lysate was performed to detect the amount of LC3 and P62 (A, B, C and D), indirect immunofluorescence (IF) analysis of the co-localization of autophagosomes (LC3, green) and lysosomes (LAMP-1, red) bar, 10 μM (E and F).

(G and H) Pearson correlation coefficients (PCCs) of images of internalized Alexa Fluor 488-LC3 and Alexa Fluor 594-LAMP1 in THP-1 cells. Data are presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, ns, not significant.