Figure 5.

NPC1 inhibits intracellular survival of BCG and Mtb

(A–J) THP-1 cells pretreated with control siRNA or NPC1 siRNA were infected with BCG or H37Rv for a specified period of time, and then CFU detection (A–D) was performed, western blotting of the lysate was performed to detect the amount of LC3 (E–H), IF analysis of the co-localization of autophagosomes (LC3, green) and lysosomes (LAMP-1, red) bar, 10 μM (I and J).

(K and L) PCCs of images of internalized Alexa Fluor 488-LC3 and Alexa Fluor 594-LAMP1 in THP-1 cells. Data are presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, ns, not significant.