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. 2022 Apr 22;25(5):104279. doi: 10.1016/j.isci.2022.104279

Figure 6.

Figure 6

Rv1759c induces the expression of miR-25 through NFKBIZ

(A–C) (A) qRT-PCR detection of the expression of miR-25 in THP-1 cells infected with H37Rv, H37RvΔphoP , H37RvΔ0818 , H37RvΔ3220c, H37RvΔ3143 or H37RvΔ1759c an MOI of 5. The H37Rv and H37RvΔ1759c were used to infect THP-1 cells at an MOI of 5 according to the abovementioned infection method, and then CFU detection was performed at 0 hpi, 4 hpi and 24 hpi (B and C).

(D) Relative expression levels of NFKBIZ from RNA-seq data.

(E) qRT-PCR detection of the fold changes of NFKBIZ relative to the CELL group. (F) qRT-PCR detection of the expression of miR-25 in THP-1 cells pre-transfected with NC siRNA or NFKBIZ siRNA and infected with H37Rv.

(G) THP-1 cells pre-transfected with NC siRNA or NFKBIZ siRNA were infected with H37Rv for a specified period of time, and then CFU detection was performed. Data are presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, ns, not significant.