Fig. 1.

Pseudovirus entry inhibition screening of drug library. A-D) 293T, 293T-ACE2, A549-ACE2 and Vero TMPRSS2 cells were transduced with SARS CoV2 pseudotyped virus particles containing luciferase reporter gene and luminescence was measured after 72 h. Bar graph shows respective Relative Light Units (RLUs) from Mock (Blue) without envelope and SARS CoV2 spike pseudotyped virus particle (Red) transduced cells. E) RLU values from 293T-ACE2 cells transduced with serially diluted SARS CoV2 spike pseudotyped virus particles. F–I) Bar graphs show RLU values from pseudovirus entry inhibition assay where 293T ACE2 cells were pre-treated with 1 μM of indicated drugs for 1 h and then transduced with SARS CoV2 spike pseudotyped virus particles. Cathepsin-L inhibitor CAS 108005943 was used as a positive control. J) Bar graph shows summary of percent pseudovirus entry inhibition values for each drug. Values represent Mean ± SEM, n = 3 and two independent experiments.