Fig. 2.

Validation of cell death inhibition assay. A) Cells were infected with serial dilutions of SARS CoV2 (10^-2 to 10^-8) and cell death was quantified by Cell-Titer-Glo cell viability assay and TCID50 for each cell line was determined at 72 h. B) Cells were pre-treated with different concentrations of Remdesivir followed by infection with 10 TCID50 of SARS CoV2 and the effect on cell death inhibition was quantified. C-E) Dose response effect of candidate drugs apilimod, berbamine, cepharanthine and (S)-crizotinib on USA/WA1 SARS CoV2 induced cell death in 293T-ACE2, Vero E6 and Vero-TMPRSS2 cells was analyzed. F) Vero TMPRSS2 cells were infected with 10TCID₅₀ SARS CoV2 and the nucleocapsid positive (NP+) cells were enumerated by flow cytometry 24 h post infection. Numbers in the histograms show the percentage of NP + cells. G) Bar graph shows the % inhibition in NP + cells in the presence of candidate drugs. Values represent Mean ± SEM, n = 3 and two independent experiments.