Table 2.
Sequence similarity between cullins in humans and Dictyostelium discoideum.
| Human protein (Uniprot ID) | Size (aa) | Dictyostelium discoideum protein (dictyBase gene ID) | Size (aa) | Region of similarity (aa) | Identities (%)* | Positives (%)** | E-value |
|---|---|---|---|---|---|---|---|
| CUL1 (Q13616) | 776 | CulA (DDB_G0291972) | 770 | 772 | 51 | 68 | 0 |
| CulB (DDB_G0267384) | 771 | 726 | 38 | 61 | 1E-136 | ||
| CulE (DDB_G0278991) | 750 | 783 | 31 | 54 | 1E-104 | ||
| CulC (DDB_G0284903) | 769 | 801 | 30 | 50 | 6E-99 | ||
| CulD (DDB_G0292794) | 802 | 801 | 28 | 47 | 4E-69 | ||
| Anapc2 (DDB_G0276377) | 907 | 226 | 22 | 41 | 6E-05 | ||
| CUL2 (Q13617) | 745 | CulA (DDB_G0291972) | 770 | 774 | 37 | 57 | 1E-135 |
| CulB (DDB_G0267384) | 771 | 777 | 35 | 57 | 1E-131 | ||
| CulC (DDB_G0284903) | 769 | 780 | 29 | 51 | 1E-87 | ||
| CulE (DDB_G0278991) | 750 | 760 | 30 | 51 | 1E-83 | ||
| CulD (DDB_G0292794) | 802 | 780 | 25 | 47 | 3E-63 | ||
| Anapc2 (DDB_G0276377) | 907 | 157 | 24 | 40 | 0.013 | ||
| CUL3 (Q13618) | 768 | CulC (DDB_G0284903) | 769 | 777 | 48 | 66 | 0 |
| CulD (DDB_G0292794) | 802 | 790 | 34 | 54 | 1E-113 | ||
| CulA (DDB_G0291972) | 770 | 725 | 32 | 52 | 2E-97 | ||
| CulB (DDB_G0267384) | 771 | 795 | 29 | 51 | 2E-86 | ||
| CulE (DDB_G0278991) | 750 | 704 | 28 | 47 | 2E-62 | ||
| Anapc2 (DDB_G0276377) | 907 | 224 | 22 | 38 | 0.25 | ||
| CUL4A (Q13619) | 759 | CulD (DDB_G0292794) | 802 | 763 | 46 | 64 | 0 |
| CulC (DDB_G0284903) | 769 | 776 | 38 | 59 | 1E-142 | ||
| CulA (DDB_G0291972) | 770 | 700 | 33 | 53 | 2E-88 | ||
| CulB (DDB_G0267384) | 771 | 741 | 27 | 49 | 2E-71 | ||
| CulE (DDB_G0278991) | 750 | 611 | 30 | 50 | 2E-61 | ||
| Anapc2 (DDB_G0276377) | 907 | 177 | 25 | 40 | 2E-04 | ||
| CUL4B (Q13620) | 913 | CulD (DDB_G0292794) | 802 | 761 | 48 | 65 | 0 |
| CulC (DDB_G0284903) | 769 | 766 | 38 | 60 | 1E-139 | ||
| CulA (DDB_G0291972) | 770 | 701 | 32 | 54 | 8E-88 | ||
| CulB (DDB_G0267384) | 771 | 741 | 26 | 48 | 7E-67 | ||
| CulE (DDB_G0278991) | 750 | 610 | 29 | 50 | 5E-59 | ||
| Anapc2 (DDB_G0276377) | 907 | 180 | 27 | 40 | 9E-05 | ||
| CUL5 (Q93034) | 780 | CulA (DDB_G0291972) | 770 | 797 | 28 | 52 | 3E-85 |
| CulB (DDB_G0267384) | 771 | 764 | 29 | 51 | 6E-84 | ||
| CulE (DDB_G0278991) | 750 | 661 | 28 | 52 | 3E-65 | ||
| CulC (DDB_G0284903) | 769 | 689 | 26 | 48 | 8E-55 | ||
| CulD (DDB_G0292794) | 802 | 674 | 26 | 47 | 1E-39 | ||
| CUL7 (Q14999) | 1,698 | CulA (DDB_G0291972) | 770 | 358 | 21 | 37 | 0.54 |
| CUL9 (Q8IWT3) | 2,517 | CulA (DDB_G0291972) | 770 | 391 | 23 | 39 | 4E-05 |
BLASTp searches were performed using dictyBase. Human proteins were used as query sequences (E-value, 1; Matrix, BLOSUM62; Filter, no).
Sheikh et al. (2015) performed a similar analysis to show that CUL1 is most similar to CulA, CulE, and CulB, CUL3 is most similar to CulC, and CUL4B is most similar to CulD.
*
Exact amino acid match.
**
Similar amino acid match (e.g., both polar).