Input and IP dilution factors

	Input dilution factor relative to IP		Input and IP individual dilution factors at each step
Step	Input	IP	Input	IP
RNA extraction starting material	×0.1	1	×0.1	×1
RNA extraction final volume	×1.212	1	×1.212	×1
DNase	TBD, maximum is ×0.991 (max = 26 μL of RNA samples = 86.67% of total volume)	1 (87.5% of total volume of RNA sample)	Max × 0.867	×0.875
RT	×0.105 (10% of DNase; 3 μL)	1 (95.71% of DNase)	×0.1	×0.957
qPCR	×0.06 (50-fold)	1 (3-fold)	×0.02	×0.33
plate	1 (5 μL/25 μL total, per well)	1 (5 μL/25 μL total, per well)	×1	×1
“Total dilution factor”	×0.00076 (7.6 × 10-4)	1	×0.00021	×0.276
IN/IP relative dilution factor∗	×0.00076 (7.6 × 10-4)		×0.00076052219 (7.6 × 10-4)

^∗For each IP, we use 0.00076-times less input (0.076%). Thus, the IP/IN ratio must be multiplied by 0.00076 ([IP value] × 1)/([input value] × 7.6 × 10^-4)]