Table 2.

Genome depth, coverage, and mapping rates of known concentrations of SARS-CoV-2 seeded in wastewater in Trials A and B. Average and range values were generated from each batch of dilution (9 samples each) in Trials A and B.

Concentrations of SARS-CoV-2 seeded/50 mL of wastewater	No. of wastewater samples analyzed		Total no.of quality-filtered reads(mean ± SD)	Total no. ofSARS-CoV-2 mapped reads(mean ± SD)	Range of SARS-CoV-2 mapping rates (%)	Average genome depth (range)	Range of genomic coverage breadth at ≥30 times the depth (%)	Mapping rate (%)
Trial A
2.32 × 105 GC	9	1.93 ± 0.06 × 107		289,875 ± 341,950	0.36 – 5.73	222 – 3214	93.9 – 99.9	92.4 – 93.8
10−1 Dilution	9	1.96 ± 0.03 × 107		4383 ± 2702	0.00 – 0.05	1.39 – 27.9	13.5 – 52.9	92.3 – 93.9
10−2 Dilution	9	1.95 ± 0.04 × 107		1271 ± 1913	0.00 – 0.30	0.01 – 19.4	1.30 – 15.6	91.6 – 94.0
10−3 Dilution	9	1.91 ± 0.19 × 107		33 ± 78	0.00 – 0.00	0.00 – 0.78	0.00 – 1.65	93.0 – 93.5
Trial B
1.79 × 105 GC	9	1.71 ± 0.44 × 107		48,013 ± 90,543	0.02 – 3.27	12.2 - 945	71.4 – 99.3	95.5 – 96.5
10−1 Dilution	9	1.79 ± 0.40 × 107		912 ± 1499	0.00 – 0.03	0.00 – 16.1	0.00 – 50.8	96.0 – 97.4
10−2 Dilution	9	1.88 ± 0.18 × 107		304 ± 321	0.00 – 0.00	0.00 – 2.89	0.00 – 9.70	95.7 – 99.1
10−3 Dilution	9	1.69 ± 0.47 × 107		94 ± 178	0.00 – 0.00	0.00 – 1.65	0.00 – 8.40	94.8 – 97.0

CCDC: China CDC; SD = standard deviation.