Table 3.
Proportion of samples positive for SARS-CoV-2 RNA in Trials A and B of wastewater seeded at four concentrations using four RT-qPCR assays and ATOPlex sequencing.
| Concentrations of SARS-CoV-2 seeded/50 mL of wastewater |
No. of wastewater samples positive/No. of samples tested (%) |
|||||
|---|---|---|---|---|---|---|
| US CDC N1 | US CDC N2 | CCDC N1 | CCDC ORF1ab | RT-qPCR all assays combined | ATOPlex | |
| Trial A | ||||||
| 2.32 × 105 GC | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) |
| 10−1 Dilution | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) |
| 10−2 Dilution | 8/9 (88.9) | 1/9 (11.1) | 9/9 (100) | 7/9 (77.8) | 9/9 (100) | 6/9 (66.6) |
| 10−3 Dilution | 6/9 (66.6) | 1/9 (11.1) | 3/9 (33.3) | 1/9 (11.1) | 8/9 (88.9) | 1/9 (11.1) |
| Trial B | ||||||
| 1.79 × 105 GC | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) |
| 10−1 Dilution | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) | 8/9 (88.8) |
| 10−2 Dilution | 8/9 (88.8) | 6/9 (66.6) | 4/9 (44.4) | 6/9 (66.6) | 8/9 (88.8) | 6/9 (66.6) |
| 10−3 Dilution | 5/9 (55.5) | 2/9 (22.2) | 1/9 (11.1) | 5/9 (55.5) | 7/9 (77.7) | 3/9 (33.3) |
CCDC: China CDC.