. 2022 Mar 29;100(5):skac095. doi: 10.1093/jas/skac095

Table 4.

Effect of treatment and stage of storage on the in vitro fermentation profile of wet brewer’s grain silage^1,2

Item³	Treatment					Mean	SEM	P-value
Item³	CON	INO	NaL1	NaL2	PRP	Mean	SEM	TRT	STG	TRT × STG
IVDMD, g/kg of DM							23.9	0.035	<0.001	0.032
Fresh	501^A	538^A	555^A	524^A	479^AB
Ensiled	421^Bc	451^Bbc	481^Babc	505^ABab	523^Aa
AES	367^Bbc	401^Babc	350^Cc	449^Ba	425^Bab
IVOMD, g/kg of OM							18.2	0.034	<0.001	0.026
Fresh	543^A	567^A	573^A	551^A	525^A
Ensiled	463^Bc	490^Bbc	510^Babc	528^Aab	545^Aa
AES	399^Cbc	438^Cab	386^Cc	468^Ba	440^Bab
pH							0.02	<0.001	<0.001	<0.001
Fresh	6.47^C	6.48^C	6.50^B	6.52^C	6.48^B
Ensiled	6.59^Bab	6.57^Bb	6.51^Bc	6.62^Ba	6.49^Bc
AES	6.70^Acd	6.74^Ac	6.81^Ab	6.86^Aa	6.69^Ad
NH₃-N, mg/dL							1.07	0.026	<0.001	0.114
Fresh	-	-	-	-	-	27.2^C
Ensiled	-	-	-	-	-	34.4^B
AES	-	-	-	-	-	40.7^A
Mean	36.2^a	35.4^a	33.9^ab	32.7^ab	30.3^b

CON, control (no additive); INO, inoculant delivering L. lactis and L. buchneri 150,000 cfu per g of fresh WBG each; NaL1, sodium lignosulfonate applied at a rate of 10 g/kg fresh basis; NaL2, sodium lignosulfonate applied at a rate of 20 g/kg, fresh basis; PRP, propionic acid (Alfa Aesar; 99%) applied at a rate of 5 g/kg, fresh basis. Fresh, fresh WBG prior to ensiling; Ensiled, ensiled WBG after 60 d of storage; AES, aerobically exposed WBG silage 10 d after opening.

Whenever the interaction TRT × STG was not significant (P > 0.05), the means of significant main effects (P ≤ 0.05) are presented instead.

IVDMD, in vitro dry matter digestibility; IVOMD, in vitro organic matter digestibility; NH₃-N, ammonia-N.

Means with different uppercase superscripts within a column are significantly different (P ≤ 0.05).

Means with different lowercase superscripts within a row are significantly different (P ≤ 0.05).