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. 2022 Mar 29;100(5):skac095. doi: 10.1093/jas/skac095

Table 5.

Effect of treatment and stage of storage on methane production and gas kinetics of wet brewer’s grain silage fermented in vitro for 24 h1,2

Item3 Treatment Mean SEM P-value
CON INO NaL1 NaL2 PRP TRT STG TRT × STG
Mf, mL/g incubated DM 7.81 <0.001 <0.001 0.058
 Fresh - - - - - 215A
 Ensiled - - - - - 181B
 AES - - - - - 155C
 Mean 184b 182bc 169c 183b 200a
Kf, %/h 0.847 0.006 <0.001 0.09
 Fresh - - - - - 14.6A
 Ensiled - - - - - 12.6B
 AES - - - - - 9.81C
 Mean 13.4a 13.0ab 11.0c 11.8bc 12.6ab
Lag, h 0.228 0.400 0.007 0.007
 Fresh 0.15B 0.274 0.522 0.498 0.448B
 Ensiled 0.148B 0.250 0.910 0.230 0.174B
 AES 1.39Aa 0.644bc 0.430c 0.230c 1.15Aab
Methane production, mg 0.270 0.058 <0.001 0.775
 Fresh - - - - - 3.16A
 Ensiled - - - - - 2.43B
 AES - - - - - 2.00C
Methane concentration, mM 0.069 0.087 0.033 0.696
 Fresh - - - - - 0.944A
 Ensiled - - - - - 0.850B
 AES - - - - - 0.833B
Methane yield, mmol/g fermented OM 0.026 0.072 0.025 0.855
 Fresh - - - - - 0.273A
 Ensiled - - - - - 0.234B
 AES - - - - - 0.233B

CON, control (no additive); INO, inoculant delivering L. lactis and L. buchneri 150,000 cfu per g of fresh WBG each; NaL1, sodium lignosulfonate applied at a rate of 10 g/kg fresh basis; NaL2, sodium lignosulfonate applied at a rate of 20 g/kg, fresh basis; PRP, propionic acid (Alfa Aesar; 99%) applied at a rate of 5 g/kg, fresh basis. Fresh, fresh WBG prior to ensiling; ensiled, ensiled WBG after 60 d of storage; AES, aerobically exposed WBG silage 10 d after opening.

Whenever the interaction TRT × STG was not significant (P > 0.05), the means of significant main effects (P ≤ 0.05) are presented instead.

Mf, asymptotic maximal gas production in a 24 h in vitro fermentation. Kf, rate of gas production.

Means with different uppercase superscripts within a column are significantly different (P ≤ 0.05).

Means with different lowercase superscripts within a row are significantly different (P ≤ 0.05).